Carcinogenesis, Teratogenesis & Mutagenesis ›› 2006, Vol. 18 ›› Issue (2): 81-083.doi: 10.3969/j.issn.1004-616x.2006.02.001

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Cloning the Gene Pancreatic Duodenal Homeobox-1 and Its Expression in SMMC-7721 Hepatoma Cells

CHEN Yuan-xiao1;LI Wen-lin2; HE Zhi-ying2;BA Yue3; TIAN Ming1;ZI Xiao-yuan2;ZHANG Yan1;HU Yi-ping2   

  1. 1. Department of Cell Biology and Genetics, Kunming Medical College, Kunming 650031，Yunnan,China；2. Department of Cell Biology, College of Basic Medical Science, Second Military Medical University, Shanghai 200433,China；3. School of Public Health, Zhengzhou University, Zhengzhou 450052,Henan,China
  • Received:2005-06-06 Revised:2005-10-25 Online:2006-03-30 Published:2006-03-30
  • Contact: HU Yi-ping

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Abstract: BACKGROUND ＆ AIM: To clone the mouse pdx-1 gene and to construct its expression vector. MATERIAL AND METHODS： Mouse pdx-1 gene was amplified from mouse pancreas total RNA and subcloned into the vectors pEGFP-C1 and pcDNA3.0. The expression of pdx-1 was analyzed by RT-PCR and green fluorescence detection after transfection of the SMMC-7721 cell line. RESULTS： The expression vectors of pEGFP-C1 pdx-1 and pcDNA3 pdx-1 were constructed. After transfecting the SMMC-7721 cells with pEGFP-C1 pdx-1, green fluorescence was detected only in the nucleoli. By RT-PCR analysis, pdx-1 mRNA was detected in both pEGFP-C1 pdx-1 and pcDNA3 pdx-1 transfected cells. CONCLUSION： The expression vectors of pdx-1 gene were useful to investigate the development of the pancreas and the proliferation and differentiation of hepatic stem cells.

Key words: pancreatic duodenal homeobox-1 (pdx-1), gene cloning, gene expression

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